Figure 2.

Patient-derived pancreatic xenograft models differ in tumor and stroma content. Classification of EF5 stained sections using the Aperio pattern recognition software. Sections of tumors were examined for (A) epithelial and (B) stromal content. Analysis of the hypoxic fraction in the (C) epithelial and (D) stromal compartment of EF5 stained sections of tumors. Stromal hypoxia, although overall displaying a lower magnitude, shows the same pattern of high hypoxic and low hypoxic models that can be established from the analysis of the epithelial hypoxia; (E) Tumor hypoxia in the epithelial compartment strongly correlated with the stromal compartment in sections stained for the hypoxia marker EF5 using the Aperio pattern recognition software. Metastatic potential (red square) to liver and peritoneum was observed in models with high tumor and stromal hypoxia.