Figure 4. Sarcoplasmic reticulum, sarcolemmal Ca2+ handling and mitochondrial function.
(A) Total SR Ca2+-content was significant lower in LCR rats compared to HCR. (B) NCX function was increased in LCR rats assessed by the rate constant of Ca2+ removal(Fura2/AM ratio340/380· second−1),(C) SERCA-2a function was reduced in LCR rats. (D) Mitochondrial respiration by ADP-stimulation of respiration to maximal respiration in freshly dissected saponin permeabilized left ventricle tissue was significantly lower in LCR rats compared to HCR (determined by µmol O2 · g−1 · min−1 consumption). (E) Exemplary recordings from LCR (red) and HCR (blue). Number of rats used for experiments: LCR=5, HCR=5.