Figure 10. Testing the model of IL-2 regulation through mixed culture of two T cell clones.
5C.C7 and A1(M) TCR transgenic T cells were cultured at varied precursor frequencies (5.103 and 5.104 T cells/well) with titrated concentrations (10−6/10−7/10−8 M) of cognate antigens (K5 and HY peptides) pulsed on separate splenocytes. Graphs show two experiments and are representative of three experiments. (A) Model predictions (top) and experimental validation (bottom) of [IL-2]max for mixed cultures. Color represents total number of T cells (5.103 + 5.103, 5.103 + 5.104, 5.104 + 5.104). (B) Model (left) and experimental (right) cumulative distributions of the ratio of [IL-2] accumulated by the mixed culture to the sum of [IL-2] accumulated independently by each clone over all conditions. (C) Model prediction (left) and experimental validation (right) that pSTAT5 response to shared IL-2 can resolve the relative activating doses of antigen for 5.104 cells of each clone. Marker shape: [Antigen1] = [K5], marker size: [Antigen2] = [HY], color: ratio [K5]/[HY].
Figure 10—figure supplement 1. Additional computational predictions and experimental validation for a mixed culture of two T cell clones.
