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. Author manuscript; available in PMC: 2014 Apr 9.
Published in final edited form as: Brain Res. 2002 Dec 27;958(2):371–380. doi: 10.1016/s0006-8993(02)03683-1

Fig. 5.

Fig. 5

Western blot analyses of TTR in immortalized choroidal epithelial cells. Cytosolic proteins from the cells of selected colonies, choroid plexus tissues, and liver homogenates, as well as proteins in culture media, were electrophoresized on 8.5% SDS–polyacrylamide gel, followed by transferred on to a PVDF membrane. Immunoblots resulting from the reaction with anti-rat TTR antibody were visualized by ECL luminescence method. TTR migrates with apparent MW of 55 KDa. Lanes: (1) rat TTR standard; (2) primary cultured plexus cells; (3): clone 3-2 (developed into Z310 cell line); (4): clone 1–2; (5) culture medium for clone 3-2; (6) culture medium for clone 1–2; (7) choroid plexus tissues; (8) liver cytosolic fractions.