Table 2.

Nucleotide sequence and amplification efficiency of 20 sugarcane SCoT primers.

Number	Primer	Sequence (5′-3′)	GC (%)	NTB	NPB	PPB	PIC
1	P1	CAACAATGGCTACCACCA	50	11	10	90.91	0.895
2	P3	CAACAATGGCTACCACCG	56	10	8	80.00	0.892
3	P6	CAACAATGGCTACCACGC	56	9	9	100.0	0.884
4	P8	CAACAATGGCTACCACGT	50	8	8	100.0	0.842
5	P11	AAGCAATGGCTACCACCA	50	10	9	90.00	0.887
6	P12	ACGACATGGCGACCAACG	61	9	8	88.89	0.864
7	P15	ACGACATGGCGACCGCGA	67	8	7	87.50	0.854
8	P17	ACCATGGCTACCACCGAG	61	9	9	100.0	0.875
9	P22	AACCATGGCTACCACCAC	56	8	7	87.50	0.867
10	P23	CACCATGGCTACCACCAG	61	9	9	100.0	0.886
11	P25	ACCATGGCTACCACCGGG	67	9	8	88.89	0.834
12	P26	ACCATGGCTACCACCGTC	61	7	6	85.71	0.840
13	P27	ACCATGGCTACCACCGTG	61	9	9	100.0	0.884
14	P28	CCATGGCTACCACCGCCA	67	5	5	100.0	0.790
15	P29	CCATGGCTACCACCGGCC	72	11	10	90.91	0.892
16	P31	CCATGGCTACCACCGCCT	67	11	10	90.91	0.907
17	P32	CCATGGCTACCACCGCAC	67	6	6	100.0	0.806
18	P35	CATGGCTACCACCGGCCC	72	7	6	85.71	0.783
19	P37	ACGACATGGCGACCAGCG	66	10	9	90.00	0.859
20	P39	AACCATGGCTACCACCGC	61	10	10	100.0	0.882
	Subtotal			176	163
	Average					92.85	0.861

Notes: NTB: number of total bands; NPB: number of polymorphic bands; PPB: percentage of polymorphic bands; PIC: polymorphism information content.