Figure 3.

SAA-induced IL-8 levels were significantly downregulated in HEC-1A cells overexpressing UG. (a) Reverse transcription-PCR of ALX mRNA in HEC-1A cells and ALX-antisense (AS-ALX) oligo-treated HEC-1A cells. The result is from a representative gel of two performed. (b) SAA-dependent IL-8 release as measured by ELISA, in HEC-1A cells, is inhibited when pretreated with ALX-antisense oligo. In HEC-1A^UG cells SAA-induced IL-8 release is significantly lower in comparison to HEC-1A. Student's t-test for SAA versus AS-ALX treated HEC-1A cells or HEC-1A^UG cells was calculated, P < 0.0001 (∗∗). Data were normalized using number of cells. Results are the mean ± SD of three separate experiments with quadruplicate determinations.