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. 2012 Dec 4;31(2):437–446. doi: 10.3892/ijmm.2012.1203

Figure 4.

Figure 4.

Effect of adenosine receptor antagonists on the cytoprotective effects of inosine. Confluent HepG2 cultures were subjected to combined oxygen-glucose deprivation (COGD, n=32) for 14 h followed by a 4-h-long re-oxygenation period. (A and B) The A1 adenosine receptor antagonist, CDPX, (C and D) the A2A adenosine receptor antagonist, CSC, (E and F) the A2B adenosine receptor antagonist, alloxazine, and the A3 adenosine receptor antagonist, (G and H) MRS 1523 were applied in the indicated concentrations (n=3) 30 min prior to the inosine or adenosine pretreatment (INO or ADE, n=12) and were present throughout the COGD period. Viability was measured by the MTT assay (A, C, E and G) and LDH activities (B, D, F and H) were measured from the cell culture supernatant. Controls (CTL, n=16) were exposed to hypoxia in complete culture medium. Data are shown as the means ± SEM. #P<0.05 compared to CTL and *P<0.05 compared to COGD. None of the adenosine receptor antagonists affected the cytoprotective effects of adenosine (data not shown) and inosine during COGD.