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. 2013 Oct 22;14(12):1084–1091. doi: 10.1038/embor.2013.164

Figure 3.

Figure 3

H2A.Z localizes to the FOS promoter. (A–C) ChIP analysis of H2A.Z (A), p400 (B) and INO80 (C) binding to the indicated regions of the FOS locus relative to the TSS in quiescent HeLa cells. Binding of H2A.Z is calculated relative to H3 ChIP. Where indicated control ChIP with IgG is shown. (D) ChIP in WT or Parp1 knockout MEFs showing binding of p400, Ino80 and PAR to the FOS −130 region at the indicated times after PMA stimulation. (E) ChIP in HeLa cells showing relative binding of H2A and H2A.Z to the FOS −130 region after siRNA-mediated depletion of INO80, p400 or GAPDH as indicated. Data in (B), (C) and (E) are presented as means±s.e.m. and are the average of at least two independent experiments performed in at least duplicate (n⩾4). Data in (D) are presented as means±s.e.m. (n=2) of two independent experiments and data in (A) are presented as means±s.e.m. of duplicate samples and are representative of four independent experiments (n=2). ChIP, chromatin immunoprecipitation; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IgG, immunoglobulin G; MEF, murine embryonic fibroblast; PMA, phorbol myristate acetate; PARP1, Poly [ADP-ribose] polymerase 1; siRNA, small interfering RNA; TSS, transcription start site; WT, wild type.