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Insertion of flexible polypeptide linkers does not disrupt DNA- and nucleosome-stimulated ATP hydrolysis. ATPase rates were measured in the presence of saturating concentrations of ATP, DNA or nucleosomes. Errors are s.d. for ISWIWt (n=3) and minimal and maximal values of two independent measurements for all other enzyme derivatives. ATPase rates in the absence of DNA were <0.02 s−1 under otherwise identical conditions (data not shown). ISWIWt, wild-type ISWI.
