Figure 3.

Insertion of a long flexible segment into the DBD linker allows the Chd1–AraC remodeller to reach farther away from the nucleosome. End-positioned nucleosomes were generated containing the 17 bp araI₁ site located +3, +13, +23, +33 or +43 bp away from the nucleosome edge. For each remodeller variant, a rate constant was calculated for each nucleosome substrate from single exponential fits to the data (n≥3). The bar graph shows rate constants, along with calculated errors of fitting, relative to Chd1_ΔNC as in Fig 2. For Chd1_ΔNC, the average k_obs for nucleosomes with araI₁ at +3 and +13 was 0.32±0.02 min⁻¹. Asterisks denote relative rate constants≤0.004. araI₁, AraC binding site; DBD, DNA-binding domain.