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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Methods Mol Biol. 2014;1124:121–158. doi: 10.1007/978-1-62703-845-4_9

Fig. 4.

Fig. 4

Ie in an inside-out patch of membrane from a human eosinophil at −60 mV. The cell was stimulated with 60 nM PMA in cell-attached patch configuration 5 min before the patch was excised. Pipette solution was 200 mM HEPES, 2 mM MgCl, 2 mM EGTA, titrated to pH 7.5 with TMAMeSO3. The bath solution was the same as the pipette solution with 50 μM GTPγS and 3 mM MgATP added. The arrows indicate the addition of 2.5 mM NADPH or 12 μM DPI.