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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Methods Mol Biol. 2014;1124:121–158. doi: 10.1007/978-1-62703-845-4_9

Fig. 6.

Fig. 6

Evaluation of Vrev using voltage ramps. Currents during voltage ramps (from +200 mV to −100 mV in 800 ms) in a human eosinophil studied in perforated-patch configuration with KMeSO4 in the pipette solution and TMAMeSO4 in the bath solution, both at pH 7.0 and both with 50 mM NH4+ to clamp pHi near pHo. The predominant conductance is due to voltage-gated proton channels, which are inhibited by Zn2+. The sequence was 1.5 mM ZnCl2, then 5 mM, then 3 mM, then washout with EGTA-containing bath solution. This cell was stimulated with PMA and the DPI was added to inhibit NADPH oxidase.