. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: Methods Mol Biol. 2014;1124:121–158. doi: 10.1007/978-1-62703-845-4_9

Table 1.

Examples of solutions used for proton channel characterization

Pipette Solutions
pH	MeSO₃	CsCl	HCl	Base	EGTA	CaCl₂	MgCl₂	Buffer
5.5	96			80 TMA	1		2	100 MES
5.5	70		10	TEA		2	1	100 MES
6	80			130 TMA	1		2	100 MES
6	70		10	TEA		2	1	100 MES
6.5	128			75 TMA	1		2	100 BIS-TRIS
6.5	70		10	TEA		2	1	100 PIPES
7	128			115 TMA	1		2	100 HEPES
7	70		10	TEA		2	1	100 HEPES
7.5		75		50 CsOH				100 HEPES
8		75		44 CsOH				100 TRIS

Bath Solutions
pH	MeSO₃	CsCl	HCl	Base	BAPTA	EGTA	CaCl₂	MgCl₂	Buffer
5.5	96			80 TMA		1		2	100 MES
6	70		10	TEA			1		100 MES
6.5	70		10	TEA	5		2		100 PIPES
6.5		35		132 CsOH	10				100 PIPES
7	70		10	TEA	5		2		100 HEPES
7.2		75		30 CsOH	10		11.4		100 HEPES

The table lists examples of solutions used for pipette and bath solutions when recording proton currents in whole cell configuration. Numbers indicate concentration in mM. Where no numbers are given the base was used to titrate the solution to the correct pH.