Table 2.
Examples of solutions used for K+ channel characterization.
| Pipette Solutions | |||||||
|---|---|---|---|---|---|---|---|
| pH | NaCl | KCl | CaCl2 | MgCl2 | HEPES | Glucose | NaOH |
| 7 | 137.6 | 5 | 2 | 1 | 10 | 6 | 2.4 |
| 7 | 126.6 | 16 | 2 | 1 | 10 | 6 | 2.4 |
| 7 | 117.6 | 25 | 2 | 1 | 10 | 6 | 2.4 |
| 7 | 42.6 | 100 | 2 | 1 | 10 | 6 | 2.4 |
| 7 | 2.6 | 140 | 2 | 1 | 10 | 6 | 2.4 |
| Bath Solutions | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| pH | NaCl | KCl | CaCl2 | MgCl2 | Buffer | BAPTA | EDTA | MgATP | Na2ATP | HCL | KOH |
| 7 | 5 | 97.6 | 0.7 | 10 HEPES | 10 | 1 | 42.6 | ||||
| 7 | 3 | 97.6 | 1.6 | 10 HEPES | 10 | 10 | 1 | 2 | 41.5 | ||
| 5.5 | 102 | 0.06 | 0.69 | 10 MES | 10 | 2.5 | 3.6 | 38 | |||
| 8 | 4 | 97.2 | 0.83 | 0.76 | 10 Taps | 10 | 0.5 | 42.6 | |||
The table lists examples of solutions used for pipette and bath solutions when recording proton currents in whole cell configuration. Numbers indicate concentration in mM.