Table 4.
Examples of solutions used for perforated patch recordings.
| Solution | TMA MESO3 | (NH4)2SO4 | MgCl2 | CaCl2 | EGTA | Buffer | pH | pHi |
|---|---|---|---|---|---|---|---|---|
| pipette solution | 100 | 25 | 2 | 1.5 | 1 | BES 10 | 7 | N/A |
| Bath 1 | 100 | 25 | 2 | 1.5 | 1 | MES 10 | 5.5 | 5.5 |
| Bath 2 | 100 | 25 | 2 | 1.5 | 1 | MES 10 | 6.5 | 6.5 |
| Bath 3 | 100 | 25 | 2 | 1.5 | 1 | BES 10 | 7.5 | 7.5 |
| Bath 4 | 100 | 25 | 2 | 1.5 | 1 | Tricine 10 | 8.5 | 8.5 |
| Bath 5 | 100 | 1.25 | 2 | 1.5 | 1 | BES 10 | 7 | 6 |
| Bath 6 | 100 | 1.25 | 2 | 1.5 | 1 | Tricine 10 | 8.5 | 7.5 |
| Bath 7 | 100 | 1.25 | 2 | 1.5 | 1 | HEPES | 7 | 6 |
The table lists examples of solutions used for pipette and bath solutions when recording proton currents in perforated patch configuration. Numbers indicate concentration in mM. The pipette solution is the same for all bath solutions. pHi is calculated from Equation 8 (see Note 7).