Figure 9.

UPR pathway analysis in wild-type and mutated hESC-CMs. (A–C) Western blot analysis of ATF6 (A), Calnexin (B), and Calreticulin (C) in eGFP⁺ and eGFP⁻ cell populations purified from differentiated wild-type and mutated hESCs. Actin is shown as a loading control. (D, E) Western blot analysis of HERG channel, calnexin, and calreticulin (D) and quantification of trafficking efficiency (E) under basal conditions (CTR) and upon proteasome (LACT) or lysosome (LEUP) inhibition in eGFP⁺ cell populations purified from differentiated wild-type and mutated hESCs. Actin is shown as a loading control. Trafficking efficiency=fg/(fg+cg), where fg=fully-glycosylated 155 kDa band and cg=core-glycosylated 135 kDa band.

Source data for this figure is available on the online supplementary information page.

Source Data for Figure 9 ^{(4.5MB, pdf)}