Figure 1.

CPT mediates apoptotic cell death. (A) Caspase-3/7 activities were measured using a luminescent assay by adding a profluorescent caspase-3/7 consensus substrate to cell lysates from p53(+) cells treated with DMSO (mock), CPT, or Nut3 for 16 h. *P<0.01; error bars represent standard deviation (s.d.). n=3. Total cell lysates from the same samples were subjected to immunoblot analysis with anti-p53 or anti-GAPDH (loading control) antibodies. (B) mRNA levels of pro-apoptotic genes (PUMA and BAX), which are transcriptional targets of p53, and anti-apoptotic Bcl-w normalized to GAPDH were monitored by qRT-PCR analysis in p53(+) cells treated with DMSO (mock), CPT, or Nut3 for 16 h.

Source data for this figure is available on the online supplementary information page.

Source Data for Figure 1 ^{(5.2MB, tif)}