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. 2013 May 28;32(13):1927–1940. doi: 10.1038/emboj.2013.124

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Copyright © 2013, European Molecular Biology Organization

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/.

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Transients pH_mito elevations in single mitochondria. (A) Time sequence of F480/F430 ratio images of HeLa cells expressing mito-SypHer showing spontaneous alkalinization transients (arrows) in single mitochondria in different cellular regions. Warm colours denote high ratio values. See also Supplementary Movie S1. (B) Repetitive pH transients in a mitochondrial cluster. Numbered insets show consecutive images and the right-hand panel shows a 30 s scan along the line drawn in inset #1, the time axis running vertically from top to bottom. (C) 3D reconstruction of the line scan image. Numbers correspond to inset panels in B. (D) Calibrated pH_mito transient. (E) Histograms showing the distribution of the amplitude, time to peak, and half-life time of 61 independent pH_mito elevations.