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. 2013 May 28;32(13):1917–1926. doi: 10.1038/emboj.2013.122

Figure 6.

Figure 6

Astrocytic TDP-43M337V overexpression alters astrocyte characteristics in transgenic rats. Gene expression was examined in cross-sections of lumbar cords. (A1–5, B1–5) Immunofluorescence staining revealed that GFAP expression was initially enhanced and later depleted in some astrocytes expressing mutant human TDP-43 (indicated by arrows). (C1–5) Representative images show that S100B expression was also altered in mutant TDP-43 transgenic rats. (D1–3) Immunofluorescence staining revealed the colocalization of S100B with human TDP-43 (hTDP43) in transgenic rats. (E1–3, F1–3, G1–3) Confocal microscopy revealed that Lcn2 was induced in GFAP–tTA#2/TRE–TDP-43M337V double transgenic rats (M337V: F1–3 and G1–3) but not in GFAP–tTA#2 single transgenic rats (tTA: E1–3). Although Lcn2 colocalized well with GFAP (F1–3), it did not fully colocalize with hTDP43 (G1–3). Arrowheads point to cells that express hTDP43 but not Lcn2, and arrows point to cells that express Lcn2 but not hTDP43 (G3). Scale bars: 100 μm (A1–5 and C1–5), 40 μm (B1–5), and 25 μm (D–G).