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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2014 Feb 7;69:357–366. doi: 10.1016/j.freeradbiomed.2014.02.002

Figure 5. EcSOD increases the rate of HIF-1α protein degradation.

Figure 5

A. AdEcSOD increases the degradation rate of HIF-1α protein. MIA PaCa-2 cells were treated with cycloheximide (100 μM) to inhibit protein synthesis. The levels of HIF-1α protein and β-actin with either AdEmpty or AdEcSOD (100 MOI) were analyzed after exposure to 4% O2. The degradation rate of HIF-1α was increased by overexpression of EcSOD.

B. EcSOD did not affect HIF-1α protein synthesis. MIA PaCa-2 cells were treated with MG-132 (10 μM) to inhibit protein degradation through the proteasome. Levels of HIF-1α and β-actin in cells treated with either AdEmpty or AdEcSOD infection were determined over a period of 2 h. The amount of HIF-1α protein was unchanged in the presence of MG-132 after treatment with EcSOD.