Table 1.
Primer pairs for canonical and dominant negative PPARG variants.
| Transcript | Oligonucleotide pairs | Size (bp) | |
|---|---|---|---|
| Forward | Reverse | ||
| tPPARG | GAGAAGGAGAAGCTGTTGGC | ATGGCCACCTCTTTGCTCT | 272 |
| PPARG1 | CGAGGACACCGGAGAGGG | TGTGGTTTAGTGTTGGCTTCTT | 69 |
| PPARG2 | TTTTAACGGATTGATCTTTTGC | AGGAGTGGGAGTGGTCTTCC | 255 |
| PPARG3 | TTCTGCTTAATTCCCTTTCC | AGGAGTGGGAGTGGTCTTCC | 194 |
| PPARG1/4 | CGAGGACACCGGAGAGGG | AGGAGTGGGAGTGGTCTTCC | 211/137 |
| tORF4 | CTTGCAGTGGGGATGTCTCA | AAACCCAAAACAACTTCCCG | 279 |
| γ1ORF4 | CGAGGACACCGGAGAGGG | AAACCCAAAACAACTTCCCG | 906 |
| γ2ORF4 | TTTTAACGGATTGATCTTTTG | AAACCCAAAACAACTTCCCG | 950 |
| γ3ORF4 | TTCTGCTTAATTCCCTTTCC | AAACCCAAAACAACTTCCCG | 889 |