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. 2014 Apr 9;9(4):e93078. doi: 10.1371/journal.pone.0093078

Figure 2. Porphyrin accumulation in transiently transfected HeLa cells with mALAS2 WT- and variant-expressing plasmids.

Figure 2

(A) HeLa cells transfected with mALAS2 variants with and without (Ψ) mutated presequences analyzed 24 hours after transfection. (B) HeLa cells transfected with mALAS2 variants with mutated presequences, in cells cultured in the absence or presence of 10 nM paclitaxel (Pct), analyzed 48 hours after transfection. Normalized PPIX fluorescence values were obtained by first dividing the mean PPIX fluorescence by the mean ZsGreen1 fluorescence for each cell population and secondly dividing by the mean PPIX fluorescence/ZsGreen1 fluorescence ratio of the pIRES2-ZsGreen1-transfected cells. Each data set represents three separate experiments ± standard deviation (*p<0.05 and **p<0.01, Student’s t-test) [a.u., arbitrary units].