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. 2014 Apr 9;9(4):e93078. doi: 10.1371/journal.pone.0093078

Table 1. Plasmid constructs used to express murine ALAS2 variants in mammalian cell lines.

Plasmid Name Mutations in
mALAS2 Presequence Mature mALAS2 Sequence
pIRES2-ZsGreen1 N/A1 N/A1
pEF21 ---2 ---2
pEF22 ---2 V423L,Y428R,P432E, R433I, G434N, E435Q and L437K
pEF23 ---2 K313A
pEF24 C11S, C38S and C70S ---2
pEF25 C11S, C38S and C70S V423L,Y428R,P432E, R433I, G434N, E435Q and L437K
pEF26 C11S, C38S and C70S K313A
pEF31 C11S, C38S and C70S R433K
1

N/A, non-applicable;

2

---, no mutations.