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. 2014 Apr 9;9(4):e92081. doi: 10.1371/journal.pone.0092081

Figure 5. si-AGR2 decreased DNP-mediated invasion and motility in 5–8F cells.

Figure 5

5–8F cells were transfected with pUprosi-AGR2 (si-AGR2) or pUprosi-mock (si-mock), and 5–8F-si-AGR2 and 5–8F-si-mock were obtained by selection for G418 resistance, and treated with DNP. A, AGR2 expression was detected using western blotting. A Boyden chamber was used to measure the invasion and motility of 5–8F-si-AGR2 and 5–8F-si-mock with or without DNP treatment. The cells that invaded the membrane were fixed with methanol and stained with hematoxylin and eosin. Random fields of view were counted to determine the number of invading cells. B, invasion of the treated cells. C, motility of the treated cells. The number of traversed cells was counted in three individual experiments and presented as the mean ± SD. *indicates P<0.05.