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. 2014 Apr 9;9(4):e94171. doi: 10.1371/journal.pone.0094171

Figure 1. Schematic diagram of plasmids used in this study.

Figure 1

(A) Ubi::FT1 construct, (B) 35S::FT1:GFP construct. Overexpression constructs were developed in binary vectors pCAMBIA1300 and pGWB5. For Brachypodium FT1, the cDNA was cloned in both vectors. For wheat FT-B1, both the coding and genomic regions were cloned in pCAMBIA1300. (C and D) Ubi::FT1 RNAi constructs. (C) The Brachypodium FT1 RNAi trigger was cloned in the pCAMBIA1300-based vector. (D) The FT1 RNAi trigger from T. monococcum was cloned in the pANDA-based vector. In both constructs, expression of the selected RNAi trigger was driven by the maize Ubiquitin promoter (Ubi). The black and gray triangles indicate the left (LB) and right (RB) border repeats respectively.