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. 2014 Apr 9;9(4):e94132. doi: 10.1371/journal.pone.0094132

Figure 5. Pharmacological inhibition of PI3K p110α and PI3K p110β impairs downstream signaling.

Figure 5

(A) Western blot analysis of basal Akt/mTOR signaling activation by detection of phosphorylated downstream proteins Akt and S6 in GBM cells following treatment with increasing concentrations of PI3K p110α-specific inhibitors YM024, PIK75, or A66 (6 h). (B) Western blot analysis of phosphorylated downstream proteins Akt and S6 protein of GBM cells following treatment with increasing concentrations of the PI3K p110β-specific inhibitor TGX221 (6 h). (C) Growth factor-induced PI3K/Akt signaling activation after pretreatment of T98G and EV7 cells with PI3K p110α-specific inhibitor YM024. (D) Growth factor-induced PI3K/Akt signaling activation after pretreatment of T98G cells with PI3K p110β-specific inhibitor TGX221.