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. 2014 Apr 9;9(4):e94460. doi: 10.1371/journal.pone.0094460

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© 2014 Sugiyama et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunoblot analyses of the proteins related to antioxidation and detoxification, Nrf2, phosphorylated Nrf2 (p-Nrf2), and Maf G for Huh7.5 and HPI cells. Fold expression of transcript (HPI/Huh7.5) in corresponding genes was shown in the right according to the expression array data. (B) Immunoblot analysis of p-Nrf2 in cytosol and nucleus fractions from the cells used in (A). GAPDH and Lamin A were used as a marker protein for cytosol and nucleus, respectively. (C) Immunoblot analyses of the HCV core and NS5A proteins, Nrf2-trarget genes, Nrf2, p-Nrf2 and Maf G for Huh7.5 and HPI cells, CuHuh7.5 cells (Huh7.5 cells simply treated with cyclosporine) and CuHPI cells (HPI cells, from which HCV was eliminated with cyclosporine). (D) Immunoblot analysis of p-Nrf2 in their cytosol and nucleus fractions from the cells used in (C).