Table 3. Effect of 10% trehalose or 10% sorbitol on the thermal unfolding temperature Tm values of Sm-TSP-2 in the presence or absence of 0.1% Brij 35 at pH 7.0 in 20 mM imidazole buffer as measured by normalized tyrosine fluorescence intensity measurements.
| Formulation | Tm ( °C) | SD | |
|---|---|---|---|
| 10.0% trehalose | 0.1% Brij 35 | 64.0 | 0.3 |
| - | 63.3 | 0.7 | |
| 10.0% Sorbitol | 0.1% Brij 35 | 64.2 | 0.4 |
| - | 63.3 | 0.3 | |