Figure 2. DNI under various stressed vs non-stressed conditions characterized by SDS-PAGE (A): Lane 1: unstressed DNI; lanes 2, 3, and 4: DNI protein stressed at 40 °C for 1, 2, and 4 weeks respectively; lanes 5, 6, and 7, DNI protein stressed at 70 °C for 1, 2, and 4 weeks respectively, by SEC-HPLC (B): black curve: unstressed DNI, red curve: DNI at 40 °C for 4 weeks, blue curve: DNI at 70 °C for 4 weeks. Duplicate measurements showed similar patterns.