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Figure 2. Immunoblot of purified rETX and its mutants with monoclonal mouse anti-rETX antibody detected using Super-ECL horseradish peroxidase. Lane 1-6, mETXH106P, mETXS111Y, mETXS111H, mETXF199H, mETXF199E, mETXS111YF199E; Lane 7, rETX; Lane 7, rETX. The pre-stained protein marker (TransGen Biotech) is indicated on the right side of the blot.
