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The effects of AngII on phospho-eNOS (P-eNOS, A) and total-eNOS (T-eNOS, B) in cardiomyocytes in the presence or absence of losartan (Los), metformin (Met), Nω-nitro-L-arginine methyl ester (L-NAME), and/or splitomicin (SP). Western blot data were normalized to actin and expressed as fold change relative to control groups. *P < 0.05, **P < 0.01 versus control; +P < 0.05 versus AngII; &P < 0.05 versus AngII+Met. n = 8–12 per group.
