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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Angiogenesis. 2013 Nov 8;17(2):419–427. doi: 10.1007/s10456-013-9400-7

Figure 3. Pre-treatment with VEGF-C increases DNA damage.

Figure 3

Radiation-induced DNA damage in LECs was detected by immunofluorescence for H2A.x 20 h after radiation. Sub-nuclear foci formed by H2A.X were imaged using confocal microscopy, scale bar 10 μm. Images shown represent LECs treated with 500 ng/ml VEGF-C (a). Dose-responsive effects were examined by pre-treating LECs with 0, 50, 100, 250, 500 or 1000 ng/ml VEGF-C. The total number of foci per nucleus was counted and averaged. Data were analyzed by ANOVA with Tukey’s Honestly-Significant-Difference Test post-hoc for each radiation dose (b). blue, DAPI; green, H2A.X; *, p<0.05 when compared to 0 ng/ml, 50 ng/ml 100 ng/ml, 250 ng/ml and 1000 ng/ml of VEGF-C; ** p<0.05 when compared to 0 ng/ml, 50 ng/ml, 100 ng/ml and 250 ng/ml of VEGF-C.