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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Med. 2014 Mar 2;20(4):377–384. doi: 10.1038/nm.3467

Figure 1. Netrin-1 and Unc5b are upregulated in obese-adipose tissue.

Figure 1

(a) Scatter plot of neuronal guidance cue expression in VAT from mice fed a HFD or chow for 20 weeks: upregulated genes are indicated in red; downregulated genes in green (n = 3 mice/group). Confirmation of mRNA changes in VAT of mice fed chow (n = 5) or HFD (n = 6) by qRT-PCR (right panel). (b) Western blot of netrin-1, Unc5b, and tubulin in VAT from mice fed chow or HFD (n = 3). (c) Relative mRNA expression of Ntn1 and Unc5b in the adipocyte and SVF fraction isolated from WAT of mice fed chow (n = 4) or HFD (n = 6). (d) Immunofluorescence staining for netrin-1 (red, upper) or Unc5b (red, lower), the macrophage marker F4/80 (green) and caveolin-1 (blue) in VAT of HFD-fed mice. Co-localization of netrin-1 or Unc5b with F4/80 is seen in yellow in the merged image (arrows). Scale bar = 100 µm. Data in a and c are the mean ± s.e.m. *P < 0.05.