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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Med. 2014 Mar 2;20(4):377–384. doi: 10.1038/nm.3467

Figure 5. Netrin-1 promotes macrophage retention in adipose tissue during obesity.

Figure 5

(a) qRT-PCR analysis of mRNA for M1 (Nos2, Tnfa, Il6) and M2 (Cd206, IL10, Pparg) markers in macrophages isolated from the VAT of mice fed chow (n = 7) or HFD (n = 9). (b) Analysis of macrophage recruitment and retention in VAT of mice fed a chow or HFD using a fluorescent bead-tracking model. Left: Representative images of fluorescent bead-labeled cells in VAT of HFD-fed mice at day 3 and 14. Scale bar = 100 µm. Right: Mean number of bead-labeled macrophages in VAT sections (n = 5 mice per group). (c) F4/80 staining (red) of VAT from HFD-fed mice 3 and 14 days post-labeling showing fluorescent beads (green) in macrophages in crown-like structures. (d) Mean number of beads in mesenteric lymph nodes on days 3 and 14 (n = 5 mice per group). Data in a, b, and d are the mean ± s.e.m. *P<0.05.