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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Med. 2014 Mar 30;20(4):443–449. doi: 10.1038/nm.3495

Figure 2. SCoRe signal is dependent on myelination.

Figure 2

(a) In vivo staining of cortical myelin with Fluoromyelin (FM) (red) labels only the reflective fibers (cyan). 10 μm scale bar. (b) In vivo FM staining in the cortex of a Thy1-YFP mouse showing that YFP-labeled axons (green), that are FM negative and therefore unmyelinated axons (arrowhead), are not reflective. Dendrites (arrow), which are never myelinated, are also not reflective. However, all FM positive segments are reflective (cyan). 10 μm scale bar. (c) Example of an axonal bifurcation in a Thy1-YFP mouse imaged in vivo demonstrating that specific parts of an axon that are FM positive are also reflective, however the unmyelinated, FM negative portions of the same axon are not reflective (arrow). 3 μm scale bar. (d) In vivo staining and imaging of myelin in the sciatic nerve with FM (red), reveals the location of nodes of Ranvier (arrows) which lack reflection (cyan). 15 μm scale bar (experiments were replicated 3 times in n=13 mice).