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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Med. 2014 Mar 30;20(4):443–449. doi: 10.1038/nm.3495

Figure 4. Multicolor reflection spectrum reveals distinct myelin structures in the spinal cord and sciatic nerve in vivo.

Figure 4

(a–b) Multicolor SCoRe images captured from the spinal cord (a) and sciatic nerve (b) showing that individual fibers reflect different colors but have a predominant color consistency along each axon. 25 μm scale bars. (c) Two differentially reflecting axons in the sciatic nerve at high-resolution. 10 μm scale bar. (d–f) In vivo SCoRe and fluorescence images captured from an mT/mG mouse expressing tdTomato in myelin sheaths (red) (d) showing Schmidt-Lanterman incisures (arrows) and nodes of Ranvier (arrowheads) (e). Combined reflection image in cyan is shown in composite with tdTomato in (f). 20 μm scale bar in d–f. (g–h) High magnification image of two Schmidt-Lanterman incisures showing SCoRe vertical interference pattern and fluorescent tdTomato (white) overlay (g) and SCoRe alone (h). (i–j) High magnification images of two Schmidt-Lanterman incisures and one node of Ranvier showing all reflected lasers (i) and combined reflection (cyan) with tdTomato fluorescence (red) (j). 4 μm scale bar in g–j (experiments were replicated 3 times in n= 8 mice for spinal cord, n=10 mice for sciatic nerve).