Figure 8.

CD34⁺ cells enhance expression of collagen 1A1 and reduce expression of matrix metalloproteinase 1 (MMP1). (A) Graphical presentation of real-time PCR analysis data of collagen 1A1 (COL1A1, left, upper panel) and MMP1 (left, lower panel) gene expressions in cultured human primary dermal fibroblasts in the presence or absence of CD34⁺ cells at various timepoints. Similar experiments were performed for expression of COL1A1 (right, upper panel) and MMP1 (right, lower panel) genes in fibroblast cells in the presence or absence of proteasome inhibitor (MG132). Internal housekeeping gene β-actin was used as a reference for normalization. Data expressed as ±SEM (n = 3 in triplicate). The expression level of each target gene in fibroblast without any treatment was considered as base line. (B) Western blot analysis was performed for C-Jun in human primary dermal fibroblast cells cultured in the presence or absence of CD34⁺ cells (1:1 ratio and CD34⁺ cells were removed after culture) plus the presence or absence of proteasome inhibitor (MG132) and JNK inhibitor II (SP600125) at various time-points. GAPDH protein was evaluated as a housekeeping control. Quantitative values of C-Jun were calculated by performing densitometric analysis of the targeted protein bands and graphically presented relative to GAPDH amount. Results are shown as mean ± SEM (n = 3) within a representative of three independent experiments.