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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Obesity (Silver Spring). 2013 Dec 5;22(5):1256–1263. doi: 10.1002/oby.20638

Figure 4. NAG-1 Tg mice on a HFD have lower inflammasome activity in WAT.

Figure 4

(A) Relative gene expression of components of the NLRP3 inflammasome: PYCARD (ASC), NLRP3, and pro-caspase-1 in the WAT of WT and NAG-1 Tg mice fed on HFD determined by Real-time PCR analysis (n=5–7 mice per group). (B) Western blot analysis of pro-caspase-1 (45kDa), cleaved-caspase-1 (10kDa), NLRP3 (NALP3) and PYCARD (ASC) in WAT of WT and NAG-1Tg mice fed on HFD. GAPDH was used as a loading control. Expressions of PYCARD and both pro- and cleaved-caspase-1 are lower in NAG-1 Tg mice compared to WT mice. PYCARD expression is lower in the WAT of NAG-1 Tg mice on HFD compared to WT mice on HFD, while NLRP3 (NALP3) expression is unchanged (data represent 4 mice per group). Data are presented as ± SE. *p<0.05, **p<0.01, and ***p<0.001 as determined by Student’s t-test.