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Characterization of PTX and siRNA co-loaded PEG-pp-PEI-PE micelles. (A) Particle size of nanoparticles determined by DLS; (B) Transmission electron microscopy (TEM) of PEG-pp-PEI-PE/PTX/siRNA by negative staining with 1% PTA; (C) Particle size distribution of PEG-pp-PEI-PE/PTX/siRNA; (D) Zeta potential of PEG-pp-PEI-PE/PTX/siRNA; (E) Stability of PEG-pp-PEI-PE/PTX/siRNA in the serum (1:10, v/v) at 37°C.
