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In vitro cellular uptake of PEG-pp-PEI-PE/siRNA. (A) Cellular uptake after MMP2 pretreatment (1h incubation in serum-free medium); (B) Cellular uptake without MMP2 pretreatment (4h incubation in 10% FBS); (C) Confocal microscopy (4h incubation in 10% FBS). Cells were stained by Hoechst 33342 and LysoTracker® Green DND-26.
