Table 2.

Box-Behnken design results for tansesterification between capric acid and mustard oil

Run	Temperature (°C)	Time (h)	Enzyme concentration (% w/w)	Speed (rpm)	Substrate Ratio	Response Incorporation (% w/w))
1	45	6	5	300	1:2	16.67
2	45	72	12	550	1:3	4.8
3	50	24	8	300	1:2	15.3
4	50	48	8	550	1:3	9.3
5	50	6	5	700	1:5	4.9
6	60	48	10	300	1:2	14.76
7	60	24	10	550	1:3	19.9
8	45	24	10	650	1:4	23.0
9	70	72	12	300	1:2	10.0
10	70	6	5	550	1:3	2.5
11	50	72	12	650	1:4	12.1
12	60	6	5	650	1:4	8.3
13	60	24	8	700	1.5	14.6
14	70	48	10	700	1:5	10.6
15	45	72	12	700	1.5	6.6