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Investigation of the neuroprotective effects of Hsp27 overexpression during chronic ethanol administration. Following the treatment of mice with 20 % ethanol for 5 weeks, degenerated neurons were detected in frozen brain sections through the use of Fluoro Jade C staining. a Representative picture of Fluoro Jade C staining in the cortex. Scale bar represents 100 μm. b Quantitative comparison of degenerated neurons. Mean ± SEM is shown; ***p < 0.001
