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. 2013 Sep 28;19(3):379–388. doi: 10.1007/s12192-013-0466-3

Fig. 3.

Fig. 3

RHBDD2 stable silencing in T47D cells. Schematic representation of the plKo system employed for stable silencing of RHBDD2 and its validation at RNA and protein level. After inserting RHBDD2 siRNA, this is transcribed in the form of shRNA by the strong U6 promoter. (a) Silencing efficiency of lentiviral particles obtained from the plkO system was evidenced at mRNA and protein levels (b)