Fig. 2.

ER stress upregulates DNAJC12 in prostate cells. LNCaP cells were exposed to 2 μM A23187 for 4 to 48 h. Whole-cell extracts were incubated with antibodies against DNAJC12 and as a loading control, the nuclear protein B23. Lane 7 contains an extract of cells transfected with a plasmid that expresses HA-tagged DNAJC12 isoform a. These results are representative of at least three independent experiments