Fig. 3.

Subcellular localization of DNAJC12. LNCaP cells that conditionally express DNAJC12HA (clone 37-3) were exposed to RSL1 and the localization of DNAJC12HA was assessed by indirect immunofluorescence using an anti-DNAJC12 antibody and Alexa Fluor 546-conjugated antibody. For comparison, DNAJC12HA-expressing cells were transfected with FLAG-tagged BiP and the localization of BiP-FLAG was assessed using an anti-FLAG antibody. The color images were converted to black and white such that DNAJC12, BiP, and cell nuclei appear in white. This figure is representative of at least three independent experiments