Table 1.

Proteins identified with ≥98 % probability in cells expressing DNAJC12HA (ordered by decreasing number of unique peptides)

Gene	UniProt	Official name or alternate designation (NCBI)	Function	NBa	Mit Locb	Uniq Pepsc	Spec (%)d
Symbol	Entry
HSPA8	P11142	Heat shock 70 kDa protein 8	Chaperone	●		21	0.29
DNAJC12	Q9UKB3	DnaJ homolog, subfamily C, member 12	Chaperone cofactor			17	1.03
PC	P11498	Pyruvate carboxylase	Glucose and lipid synthesis	●	●	10	0.08
HSP90AB1	P08238	Heat shock protein 90 kDa alpha, class B member 1	Chaperone	●		7	0.05
IDH2	P48735	Isocitrate dehydrogenase 2 (NADP+), mitochondrial	Intermediary metabolism		●	6	0.04
ATP5B	P06576	ATP synthase subunit beta, mitochondrial	ATP synthesis	●	●	5	0.03
HSPA4	O14992	Heat shock 70 kDa protein 4	Chaperone	●		5	0.03
HSPD1	P10809	Heat shock 60 kDa protein 1	Chaperone	●	●	5	0.03
GAPDH	P04406	Glyceraldehyde-3-phosphate dehydrogenase	Glycolysis			4	0.03
UQCRC2	P22695	Ubiquinol-cytochrome c reductase core protein II	Respiration		●	4	0.03
ALDH1B1	P30837	Aldehyde dehydrogenase 1 family, member B1	Alcohol metabolism		●	3	0.02
CLPX	O76031	ClpX caseinolytic peptidase X homolog	Chaperone	●	●	3	0.02
EEF2	P13639	Eukaryotic translation elongation factor 2	Protein biosynthesis	●		3	0.02
HSPA1A	P08107	Heat shock 70 kDa protein 1A	Chaperone	●		3	0.02
MTHFD1	P11586	Cytoplasmic C-1-tetrahydrofolate synthase	Tetrahydrofolate metabolism	●	●	3	0.02
SLC25A3	Q53HC3	Solute carrier family 25 member 3, mitochondrial	Phosphate carrier		●	3	0.02
	B3KUD7	FLJ39640, Highly similar to MCM7	DNA replication (presumed)			3	0.02
EEF1G	P26641	Eukaryotic translation elongation factor 1 gamma	Protein biosynthesis			2	0.03
ACADSB	P45954	Acyl-CoA dehydrogenase, short/branched chain	Dehydrogenase		●	2	0.02
ETFA	P13804	Electron-transfer-flavoprotein, alpha polypeptide	Electron transport		●	2	0.02
SEC61A1	P61619	Sec61 alpha 1 subunit	Protein transport			2	0.02
SLC25A5	P05141	Solute carrier family 25 member 5	Adenine nucleotide translocator		●	2	0.02
TIMM50	Q0VAB1	Translocase of inner mitochondrial membrane 50 homolog	Translocase		●	2	0.02
ALDH18A1	Q3KQU2	Aldehyde dehydrogenase 18 family, member A1	Amino acid synthesis	●	●	2	0.01
ATL3	Q6DD88	Atlastin GTPase 3	ER/Golgi organization	●		2	0.01
HSD17B4	P51659	Hydroxysteroid (17-beta) dehydrogenase 4	Fatty acid metabolism			2	0.01
HSPA9	P38646	Heat shock 70 kDa protein 9	Chaperone	●	●	2	0.01
NT5DC2	Q9H857	5′-nucleotidase domain-containing 2	Hydrolase			2	0.01
PFKL	P17858	Phosphofructokinase, liver	Glycolysis	●		2	0.01
PYCR2	Q96C36	Pyrroline-5-carboxylate reductase family, member 2	Amino acid synthesis		●	2	0.01
PYCRL	Q53H96	Pyrroline-5-carboxylate reductase-like	Amino acid synthesis			2	0.01
SUCLG1	P53597	Succinate Co-A ligase, alpha subunit	ATP/GTP synthesis	●	●	2	0.01
TRIM28	Q13263	Tripartite motif containing 28	Transcriptional regulation			2	0.01
	Q68CW5	DKFZp762A1314	Unknown			2	0.01
ARF3	P61204	ADP-ribosylation factor 3	Protein trafficking	●		1	0.01
MVP	Q14764	Major vault protein	Signal transduction			1	0.01
SHMT2	P34897	Serime hydroxymethyltransferase 2, mitochondrial	Amino acid synthesis		●	1	0.01
CDK1	P06493	Cyclin-dependent kinase 1	Cell cycle regulation	●		1	<0.01
EIF4A1	P60842	Eukaryotic translation initiation factor 4A1	Protein biosynthesis	●		1	<0.01
ETFB	P38117	Electron-transfer-flavoprotein, beta polypeptide	Electron transport		●	1	<0.01
HIP1R	O75146	Huntingtin interacting protein 1 related	Endocytosis			1	<0.01
IARS2	Q9NSE4	Isoleucyl-tRNA synthetase 2, mitochondrial	Protein biosynthesis	●	●	1	<0.01
PKM	P14618	Pyruvate kinase, muscle	Glycolysis	●	●	1	<0.01
PSMC6	P62333	Proteasome 26S subunit, ATPase, 6	Protein degradation	●		1	<0.01
TKT	P29401	Transketolase	Glycolysis			1	<0.01

Dots in the column labelled NB indicate that the gene encodes a nucleotide‐binding protein; Dots in the column labelled Mit Loc encode proteins that localize to mitochondria

^aNucleotide binding

^bMitochondrial localization

^cNumber of unique peptides

^dPercentage of total spectra