Figure 4.

SB431542, a pharmacological transforming growth factor-β (TGF-β)/activin receptor-like kinase 5 (ALK5) inhibitor, increases organic anion transporting polypeptide 1a4 (Oatp1a4) expression in rats subjected to hypoxia/reoxygenation (H/R). Western blot analysis of rat brain microvessels isolated from H/R rats treated with SB431542 and compared with normoxic controls. Brain microvessel membrane samples (10 μg) were resolved on a 4% to 12% SDS-polyacrylamide gel, transferred onto a polyvinylidene difluoride (PVDF) membrane, and analyzed for expression of Oatp1a4 (using the M-50 polyclonal antibody). Nx=Normoxia; Hx=Hypoxia; SB=SB431542. Results are expressed as mean±s.d. of three separate experiments where each group consists of pooled microvessels from three individual animals. Asterisks represent data points that are significantly different from control.