Figure 5.

Effects of inflammation with accumulation of activated microglia on ¹¹C-PBR28 binding (A), regional cerebral metabolic rate of glucose (rCMRglc) (B), regional cerebral metabolism of oxygen (rCMRO₂) (C), ¹¹C-flumazenil (¹¹C-FMZ) binding (D), and ¹⁸F-BCPP-EF binding (E). Regions of interest (ROIs) of inflammatory (ROI_PBR) and infarct (ROI_Infarct) regions at Day-7 (F) were placed on each quantitative image of rCMRglc, ¹¹C-FMZ, rCMRO₂, and ¹⁸F-BCPP-EF, and the ischemic/intact ratios were calculated for each probe (I). Data are expressed as mean±s.d. for seven animals; ^#P<0.05, *P<0.01 (G). After positron emission tomography (PET) imaging, monkey brains were dissected for immunohistological analyses with anti-Iba 1 (G) and anti-NeuN (H) antibodies. ¹⁸F-BCPP-EF, ¹⁸F-2-tert-butyl-4-chloro-5-{6-[2-(2-fluoroethoxy)-ethoxy]-pyridin-3-ylmethoxy}-2H-pyridazin-3-one.