Table 1. Total amounts, ¹³C labeled isotopomers and % enrichment in glucose, lactate, and alanine.

Amounts (nmol/g tissue) or % excess enrichment (%)	Sham, n=8		HI, n=9		Si versus HIi	Sc versus HIc	Si versus Sc	HIi versus HIc
	Ipsilateral	Contralateral	Ipsilateral	Contralateral
Glucose
Total	1,577±139	1,551±121	2,234±163	2,525±137	**	***		*
[1,2-13C]	621±43	622±36	783±46	897±49	*	***		0.07
% [1,2-13C]	34.5±1.1	35.5±0.9	31.6±1.3	31.7±1.0		*
Lactate
Total	2,220±149	2,206±456	2,617±238	2,423±529		0.08
[2,3-13C]	160.9±5.1	156.6±7.8	167.0±18.5	145.4±9.2
[3-13C]	6.5±0.8	5.8±0.7	1.1±0.6	4.1±1.2	***
% [2,3-13C]	7.38±0.30	7.57±0.24	6.42±0.52	6.11±0.38		**
% [3-13C]	0.29±0.04	0.28±0.03	0.04±0.02	0.16±0.05	***	0.07
Alanine
Total	588±51	554±33	1,692±197	1,033±101	**	**		**
[2,3-13C]	36.5±0.7	35.1±1.0	68.7±9.3	40.7±3.0	**			**
[3-13C]	0.9±0.2	1.1±0.2	0.5±0.3	0.0±0.0		**
% [2,3-13C]	6.48±0.49	6.49±0.42	4.18±0.45	4.27±0.53	**	**
% [3-13C]	0.18±0.05	0.21±0.04	0.04±0.02	0.01±0.00	*	**

NMRS, nuclear magnetic resonance spectroscopy; P7, postnatal day 7.

P7 rats were injected with [1,2-¹³C]glucose and brain extracts were analyzed using ¹H-NMRS (total amounts) and ¹³C-NMRS (labeled amounts). For more details, see Materials and Methods. Results are presented as mean±s.e.m. Animals subjected to hypoxia-ischemia (HI) were compared with sham-operated animals (ipsilateral versus ipsilateral and contralateral versus contralateral) using an unpaired Student's t-test while ipsi- and contralateral hemispheres within each animal were compared using a paired Student's t-test. P values are given in the four columns to the right: Si versus HIi=ipsilateral sham versus ipsilateral HI; Sc versus HIc=contralateral sham versus contralateral HI; Si versus Sc=ipsi- versus contralateral hemisphere in sham; HIi versus HIc=ipsi- versus contralateral hemisphere in HI. Near significant P values (<0.1) are given in numbers. *P<0.05, **P<0.01, ***P<0.001.