Table 2. Total amounts of central metabolites.

Amounts (nmol/g tissue)	Sham, n=8		HI, n=9		Si versus HIi	Sc versus HIc	Si versus Sc	HIi versus HIc
	Ipsilateral	Contralateral	Ipsilateral	Contralateral
Glutathione	904±27	836±25	824±36	877±28	0.10		*
Taurine	7,532±206	7,482±222	7,370±205	7,875±223				*
Creatinea	5,045±168	4,910±66	4,724±152	5,087±70		0.09		*
Myo-inositola	2,724±316	2,180±62	2,151±73	2,621±252				0.08
NAAa	1,718±69	1,658±38	1,611±70	1,691±46
Succinatea	138±10	122±3	127±5	133±6
Fumaratea	31±3	30±2	29±1	34±2				0.09
Glutamate	4,351±175	4,117±161	3,836±142	4,520±175	*			*
GABA	906±33	887±67	1,327±105	970±147	**			**
Glutamine	2,658±39	2,554±37	2,423±83	2,654±69	*			**
Aspartate	1,978±78	1,887±28	1,640±57	1,716±112	**
Serine	859±20	808±23	1,023±67	829±33	*			**
Glycine	585±9	565±15	725±39	524±18	**			**
Threonine	601±14	572±11	755±25	616±23	***			***
Tyrosine	213±21	202±21	391±55	207±17	**			**
Tryptophan	33±1	33±2	64±6	38±2	***	0.06		***
Methionine	21±2	19±1	37±6	16+1	*			**
Valine	27±2	26±2	108±20	33±1	**	**		**
Phenylalanine	33±3	32±2	86±9	45±3	***	**		**
Isoleucine	21±2	21±2	45±6	22±0	**			**
Leucine	72±10	68±7	158±26	90±6	*	*
Lysine	101±5	99±5	243±31	134±5	**	***		**

HI, hypoxia-ischemia; HPLC, high-performance liquid chromatography; NMRS, nuclear magnetic resonance spectroscopy; P7, postnatal day 7; NAA, N-acetyl aspartate.

Brain extracts of ipsi- and contralateral hemispheres from P7 rats subjected to HI were analyzed using ¹H-NMRS and HPLC. For more details, see Materials and methods. Results are presented as mean±s.e.m. P values are given in the four columns to the right: Si versus HIi=ipsilateral sham versus ipsilateral HI; Sc versus HIc=contralateral sham versus contralateral HI; Si versus Sc=ipsi- versus contralateral hemisphere in sham; HIi versus HIc=ipsi- versus contralateral hemisphere in HI. The near significant P values (<0.1) are given in numbers. *P<0.05, **P<0.01, ***P<0.001.

^aIndicates that the results are from ¹H-NMRS.